One essential step in the preprocessing of single-cell RNA-Seq data (scRNA-seq) is batch effect correction. However, much confusion remained around this step. In this article, let’s address the most frequently asked questions about handling batch effect in single-cell RNA-Seq. . What is Batch Effect in Single-cell RNA-Seq? Batch effect happens […]
Assigning cell type identity to cells is a basic yet vital step required in single-cell RNA Sequencing data analysis (scRNA-Seq), often done after dimensionality reduction and scRNA-Seq clustering . If you have successfully captured informative clusters, it’s time to face an even harder challenge: identify what cell type or cell […]
Clustering is The Microscope For scRNA-Seq data In previous posts, we have walked you through important steps in analyzing your single-cell RNA sequencing (scRNA-Seq) data, including data visualization and normalization. This time, let’s explore the next logical step in the data analysis pipeline: clustering scRNA-Seq data. What is scRNA-Seq clustering? […]
