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Tag Archives: single-cell

The Art of Setting Single-cell Quality Control Parameters
single cell qc Picture1

Performing single-cell quality control is a vital step in the data analysis pipeline. Setting QC parameters can significantly affect downstream analysis: too permissive QC filtering makes the dataset too noisy to read, and too stringent QC thresholds may remove important information.  In this article, let’s discuss the art of setting […]

The Basics of DESeq2 – A Powerful Tool in Differential Expression Analysis for Single-cell RNA-Seq
deseq2 single cell example

Differential expression analysis is a common step in a Single-cell RNA-Seq data analysis workflow. In our previous post, we have given an overview of differential expression analysis tools in single-cell RNA-Seq. This time, we’d like to discuss a frequently used tool – DESeq2 (Love, Huber, & Anders, 2014).  According to […]

Single-cell News and Views: March 2019

1. An atlas of acute myeloid leukemia at single-cell resolution Adapted from Galan et. al., 2019 Acute myeloid leukemia (AML) is a cancer characterized by the accumulation of white blood cells in the bone marrow and blood. The disease has been notoriously challenging to study because of its extreme intratumoral […]