Assigning cell type identity to cells is a basic yet vital step required in single-cell RNA Sequencing data analysis (scRNA-Seq), often done after dimensionality reduction and scRNA-Seq clustering . If you have successfully captured informative clusters, it’s time to face an even harder challenge: identify what cell type or cell […]
Single-cell RNA sequencing technologies have enabled many exciting discoveries of novel cell types and sub-types, such as the rosehip neurons (Boldog et al., 2018), disease-associated microglia (Keren-Shaul et al., 2017) and lipid-associated macrophages (Jaitin, Adlung, Thaiss, Weiner and Li et al., 2019). While sub-clustering cell populations is essential to find […]
Seurat (Butler et. al 2018) and Scanpy (Wolf et. al 2018) are two great analytics tools for single-cell RNA-seq data due to their straightforward and simple workflow. However, for those who want to interact with their data, and flexibly select a cell population outside a cluster for analysis, it is […]
